goat anti-mouse fluorescent (cy3) secondary antibodies  (Jackson Immuno)

Journal: Neural Regeneration Research

Article Title: Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

doi: 10.4103/NRR.NRR-D-23-01035

Figure Lengend Snippet: Effect of simulated weightlessness on Müller cell reverse differentiation. Immunofluorescence double staining of the rat retina. Red indicates the Müller cell marker CRALBP (Cy3), green indicates the progenitor cell marker SOX2 or PAX6 (Alexa Fluor TM 488), and blue indicates nuclear DAPI staining. (A) Representative images of CRALBP/SOX2 immunofluorescence. (B) CRALBP/SOX2-positive cells. There were more CRALBP/SOX2-positive cells in the TS4W group than in the CON4W group. (C) Representative immunofluorescence image of CRALBP/PAX6 staining. Scale bar: 20 µm (left four columns), 50 µm (rightmost column). (D) Quantification of CRALBP/PAX6-positive cells. There were more CRALBP/PAX6-positive cells in the TS4W group than in the CON4W group. Data are expressed as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01 (independent samples t -test). CON: Control; CRALBP: cellular retinaldehyde-binding protein; DAPI: 4′,6-diamidino-2-phenylindole; GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer; PAX6: paired box gene 6; TS4W: tail suspension for 4 weeks; TS8W: tail suspension for 8 weeks.

Article Snippet: Afterward, the slides were rinsed three times in PBS, and the sections were then incubated with Cy3 fluorescence-labeled goat anti-mouse IgG secondary (1:10,000, Proteintech, Cat# SA00013-4, RRID: AB_2810984) and Alexa Fluor TM 488 fluorescence-labeled goat anti-rabbit IgG secondary antibodies (1:10,000, Proteintech, Cat# SA00013-2, RRID: AB_2797132) at 37°C for 2 hours.

Techniques: Immunofluorescence, Double Staining, Marker, Staining, Control, Binding Assay, Suspension

Journal: Neural Regeneration Research

Article Title: Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

doi: 10.4103/NRR.NRR-D-23-01035

Figure Lengend Snippet: Effect of simulated weightlessness on Müller cell neural differentiation. Immunofluorescence double staining of the rat retina. Red indicates the retinal Muller cell marker CRALBP (Cy3), green indicates the retinal progenitor cell marker Crx or rhodopsin (Alexa Fluor TM 488), and blue indicates nuclear DAPI staining. (A) Immunofluorescence images of Crx staining. (B) Quantification of Crx-immunopositive cells. There were more Crx-immunopositive cells in the TS group than in the CON group. (C) Rhodopsin immunofluorescence images. Scale bar: 20 µm (left four columns), 50 µm (rightmost column). (D) Quantification of rhodopsin-immunopositive cells. There were more rhodopsin-immunopositive cells in the TS4W group than in the CON4W group. Data are expressed as mean ± SD ( n = 4 per group). ** P < 0.01 (independent samples t -test). CON: Control; Crx: cone-rod homeobox; DAPI: 4′,6-diamidino-2-phenylindole; CRALBP: cellular retinaldehyde-binding protein; TS4W: tail suspension for 4 weeks; TS8W: tail suspension for 8 weeks.

Article Snippet: Afterward, the slides were rinsed three times in PBS, and the sections were then incubated with Cy3 fluorescence-labeled goat anti-mouse IgG secondary (1:10,000, Proteintech, Cat# SA00013-4, RRID: AB_2810984) and Alexa Fluor TM 488 fluorescence-labeled goat anti-rabbit IgG secondary antibodies (1:10,000, Proteintech, Cat# SA00013-2, RRID: AB_2797132) at 37°C for 2 hours.

Techniques: Immunofluorescence, Double Staining, Marker, Staining, Control, Binding Assay, Suspension

Journal: Neural Regeneration Research

Article Title: Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

doi: 10.4103/NRR.NRR-D-23-01035

Figure Lengend Snippet: Simulated weightlessness can lead to Müller cell gelatinization. (A) Representative images of GFAP immunofluorescence (green, stained with Alexa Fluor TM 488). (B) Quantification of GFAP-immunopositive cells. There were more GFAP-immunopositive cells in the TS group than in the CON group. (C) Representative immunofluorescence images of GS (red, stained with Cy3). Scale bar: 50 µm. (D) Quantification of GS-immunopositive cells. There were more GS-immunopositive cells in the CON group than in the TS group. (E) Western blot of GFAP, GS, and GLAST. (F–H) Quantification of GFAP, GS, and GLAST relative protein expression. Data are expressed as mean ± SD ( n = 4 per group). * P < 0.05, ** P < 0.01 (independent samples t -test). CON: Control; DAPI: 4′,6-diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-photosphate dehydrogenase; GCL: ganglion cell layer; GFAP: glial fibrous acidic protein; GLAST: L-glutamate/L-aspartate transporter; GS: glutamine synthetase; INL: inner nuclear layer; ONL: outer nuclear layer; TS: tail suspension.

Article Snippet: Afterward, the slides were rinsed three times in PBS, and the sections were then incubated with Cy3 fluorescence-labeled goat anti-mouse IgG secondary (1:10,000, Proteintech, Cat# SA00013-4, RRID: AB_2810984) and Alexa Fluor TM 488 fluorescence-labeled goat anti-rabbit IgG secondary antibodies (1:10,000, Proteintech, Cat# SA00013-2, RRID: AB_2797132) at 37°C for 2 hours.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Control, Suspension